The principle aim of the course is to provide students with the skills to perform qualitative and quantitative analysis according to codified procedures, using the most common instrumental techniques. The main skills acquired will be: • To know the principles, the instrumentation of the most common spectroscopic techniques to perform qualitative and quantitative analyzes; • To know the chromatographic separation techniques and the different chromatographic retention mechanisms; • To know the instrumentation for gas chromatography and for liquid chromatography; • To know the principles of nuclear spectroscopy techniques and know how to analyze spectra related to this technique; • To know the quality control standards for the validation of a method.
Course Prerequisites
the student must have clear the concepts of molecular bonds, molecular weight, functional groups and polarity of the molecules, concepts provided in the previous courses of general and inorganic chemistry and organic chemistry
Teaching Methods
Frontal lesson
Assessment Methods
The exam includes written questions of exercises and theory
Contents
The spectroscopy module within the course of Analytical Chemistry aims to introduce problems related to NMR spectroscopy and to mass spectrometry, both techniques of considerable importance in the preparation of a future biotechnologist.
A) Nuclear magnetic resonance spectroscopy - Principles of nuclear magnetic resonance - Nuclear magnetic resonance for hydrogen - Nuclear magnetic resonance for carbon in continuous wave - Magnetic resonance in Fourier transform - Two-dimensional magnetic resonance techniques - Analysis of one-dimensional NMR spectra - Molecular recognition exercises through NMR spectra - B) Mass spectrometry - Principles of mass spectrometry - Instrumentation for mass spectrometry - Analysis of mass spectrometry spectra C) IR and UV spectroscopy
Course Language
ITALIAN
More information
Upon appointment via email using, preferably, the official student’s address: @studenti.uninsubria.it
