Effect of Electrode Distance in Electrochemotherapy: From Numerical Model to in Vitro Tests

In this paper, the electric field generated in a conductive medium by needles pairs with inter-needle distance of 1 or 2 cm has been studied by means of 3D numerical models. The computational results have been correlated with experimental ones obtained in vitro on cultured cells. In particular, electroporation effect has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. These results are helpful to evaluate the effect of the needle distance on the electric field distribution in cultured cells in terms of occurrence of reversible or irreversible electroporation.