ID:
SCV0815
Durata (ore):
54
CFU:
6
Anno:
2024
Dati Generali
Periodo di attività
Secondo Semestre (24/02/2025 - 20/06/2025)
Syllabus
Obiettivi Formativi
The aim of the course is to introduce the biological and molecular aspects of staminal cells, and their derivatives, to explore how these mechanisms lead and support the study and cure some disorders. Emphasis will be given to the inherent potential of devices, comprised of biocompatible 3D scaffolds associated with cells and their derivatives, in regenerative medicine application.
This course will also provide the students the opportunity to grow up as learners and as effective contributors by:
1. be responsible for their own learning and for the depth of their own study
2. to take advantage of all the available resources to integrate the lecture materi-al and academic knowledge.
3. Working in a team, in particular during laboratory experiences
4. Developing communication skills by effective interaction with peers and academic staff.
This course will also provide the students the opportunity to grow up as learners and as effective contributors by:
1. be responsible for their own learning and for the depth of their own study
2. to take advantage of all the available resources to integrate the lecture materi-al and academic knowledge.
3. Working in a team, in particular during laboratory experiences
4. Developing communication skills by effective interaction with peers and academic staff.
Prerequisiti
In order to follow the course, adequate knowledge of cytology, molecular biology, biochemistry and physiology is required.
Metodi didattici
The course provides lectures-style instruction and laboratory experiences. Labor-atory experiences are organized to adopt the rules to work independently, in safety and as a team.
Verifica Apprendimento
For the scaffold development section, students will be evaluated on the STL file generated and on the machine parameters defined for scaffold fabrication. Alternative designs and materials are welcome, provided that the choices are well-documented and supported by scientific or technical rational.
Oral exam and discussion of the report on the laboratory activity. The students need to demonstrate sufficient knowledge of the topics presented during the course. They will also be evaluated for their ability to present a short production (pptx is welcome), based on the consulting of research articles that they have freely consulted and concerning a topic related to the contents of the course. Basic knowledge and presentation will contribute for 90% of the final score. The report will contribute for 10% of the final score. Minimal score is fixed at 18/30.
Oral exam and discussion of the report on the laboratory activity. The students need to demonstrate sufficient knowledge of the topics presented during the course. They will also be evaluated for their ability to present a short production (pptx is welcome), based on the consulting of research articles that they have freely consulted and concerning a topic related to the contents of the course. Basic knowledge and presentation will contribute for 90% of the final score. The report will contribute for 10% of the final score. Minimal score is fixed at 18/30.
Contenuti
1- Lectures (36 h)
Section 1:
- Stem cells: an overview
- The biology of embryonic and adult stem cells.
- Cells as models for in vitro and in vivo experiments to study diseases
- Stem cells and their derivatives for regenerative medicine applications
- Cell-based drug delivery systems
- Bioethics: pros and cons in stem cell applications
Section 2:
- Introduction to 3D printing technologies and their biomedical applications.
- Overview of available 3D printing technologies: Fused Deposition Modeling (FDM), Stereolithography (SLA), Digital Light Processing (DLP), and Selective Laser Sintering (SLS).
- Survey of biocompatible and bioresorbable printing materials used in tissue engineering (e.g., PLA, PCL, hydrogels, and composites).
- Basics of CAD software for 3d modelling and scaffold design.
- Practical exercise: designing a simple scaffold structure.
- Analysis and optimization of key printing parameters (e.g., layer height, infill density, print speed, temperature).
- Simulation of 3d printing processes to predict scaffold performance.
2- Laboratory experiences (18 h)
The practical lessons consist of:
- isolation of mesenchymal stem cells from adipose tissue
- immunostaining to assess cell stemness
- preparation and accomplishment of a viability assay of cells seeded on the printed 3D scaffold
- 3d scaffold printing, post-processing, and quality evaluation.
Section 1:
- Stem cells: an overview
- The biology of embryonic and adult stem cells.
- Cells as models for in vitro and in vivo experiments to study diseases
- Stem cells and their derivatives for regenerative medicine applications
- Cell-based drug delivery systems
- Bioethics: pros and cons in stem cell applications
Section 2:
- Introduction to 3D printing technologies and their biomedical applications.
- Overview of available 3D printing technologies: Fused Deposition Modeling (FDM), Stereolithography (SLA), Digital Light Processing (DLP), and Selective Laser Sintering (SLS).
- Survey of biocompatible and bioresorbable printing materials used in tissue engineering (e.g., PLA, PCL, hydrogels, and composites).
- Basics of CAD software for 3d modelling and scaffold design.
- Practical exercise: designing a simple scaffold structure.
- Analysis and optimization of key printing parameters (e.g., layer height, infill density, print speed, temperature).
- Simulation of 3d printing processes to predict scaffold performance.
2- Laboratory experiences (18 h)
The practical lessons consist of:
- isolation of mesenchymal stem cells from adipose tissue
- immunostaining to assess cell stemness
- preparation and accomplishment of a viability assay of cells seeded on the printed 3D scaffold
- 3d scaffold printing, post-processing, and quality evaluation.
Lingua Insegnamento
INGLESE
Altre informazioni
The teachers receives the students by appointment via e-mail (rosalba.gornati@uninsubria.it; pieroantonio.zecca@uninsubria.it; ludovica.barone@uninsubria.it).
Corsi
Corsi
Biotechnology for the Bio-based and Health Industry
Laurea Magistrale
2 anni
No Results Found
Persone
Persone (3)
Assegnisti
Docenti di ruolo di Ia fascia
Ricercatori a tempo determinato
No Results Found