Quantitative PCR. Based on the principle of sample partitioning and the application of Poisson statistics, digital PCR enables absolute and accurate quantification of target molecules in biological samples. Starting from different types of samples, such as cells, tissues, and biological fluids, it allows quantification of transcript copy numbers, including microRNAs, analysis of copy number variations (CNVs) and gene amplifications, as well as detection and quantification of single nucleotide variants (SNVs). It can also be used for the identification and quantification of pathogens. Owing to its high sensitivity, reaching approximately 0.01%, digital PCR is particularly useful for the assessment of minimal residual disease in the oncological setting.
Acquisition Date:
(February 2, 2015 - )