Comparative evaluation of the bruker biotyper and Vitek MS Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance

We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. Correct identification rate at the species level was comparable using the commercial databases (89.8% vs. 84.3% p=0.712), but higher for BMS using an in-house-extended database (100% vs. 84.3% p=0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% vs. 12.1% p<0.0001), including the rate of major errors (0% vs. 4.5% p=0.0036).