Determination of mitochondrial number and function

Mitochondria transplantation is considered a potential therapeutic method that can be used in diseases related to mitochondrial dysfunction, such as neurodegenerative diseases, toxic lesions, ischemia, and cardiovascular diseases. To achieve successful transplantation, whether in vivo or in vitro, it is of paramount importance to accurately determine the number and quality of isolated mitochondria. The structural integrity of isolated mitochondria must be carefully evaluated, as it directly correlates with the organelles' ability to maintain transmembrane potential and thus promote their bioenergetic function by effectively synthesizing ATP.