The course aims to provide knowledge of the main pathogenetic mechanisms currently used in pathological anatomy laboratories for the diagnosis, prognosis, and therapy of the main neoplastic and non-neoplastic diseases.
Course Prerequisites
It is necessary to have acquired basic knowledge of Biochemistry and Genetics.
Teaching Methods
Lectures (Frontal Teaching) and the use of quick polls (even anonymous) to check the comprehension of basic concepts.
Assignment of recent scientific articles on a specific topic, followed by a request for critical summaries from students to encourage discussion.
Group discussion of clinical cases supported by group brainstorming.
Assessment Methods
Written Examination (Main Assessment) The written exam includes:
Essay/Open-ended Questions to evaluate students' conceptual linkage ability and mastery of complex topics.
Multiple-choice Questions to check basic knowledge, terminology, and technical details.
Problem-Solving Questions involving the presentation of simulated data or laboratory results.
Continuous Assessment and Interaction (During the Course) This includes:
Direct Questions asked during lectures.
Discussion of scientific articles in a group setting.
Practical Deepening Analysis of an Extended Clinical Case Study that requires students to connect theory to diagnostic practice.
Contents
Pre-Analytical Quality: From Collection to Extraction
Targeted Techniques and Precision Quantitative Analysis
NGS: Fundamentals, Platforms, and Library Preparation
NGS Analysis of Mutations, CNVs, and RNA Techniques
Liquid Biopsy (ctDNA): Test Execution (or Performing the Test)
