ID:
MED0533-5
Duration (hours):
12
CFU:
1
SSD:
SCIENZE INFERMIERISTICHE GENERALI, CLINICHE E PEDIATRICHE
Year:
2025
Overview
Date/time interval
Primo Semestre (06/10/2025 - 23/01/2026)
Syllabus
Course Objectives
- Know the main types of biological samples (urine, feces, sputum, blood, swabs, semen, etc.)
- Apply sampling techniques correctly, respecting the type of analysis (chemical-physical, cultural, cytological, etc.) and adhering to the required guidelines and protocols.
- Ensure the integrity and quality of the sample collected through appropriate collection, storage and transport, avoiding contamination, alterations and deterioration.
- Use devices and instruments safely and effectively, with particular attention to the use of needles, test tubes, sterile containers, and personal protective equipment (PPE).
- Apply safety and biological risk prevention regulations, protecting yourself, the patient, and the work environment from exposure to potential hazards.
- Observe the principles of asepsis and antisepsis to reduce the risk of sample contamination and reduce the risk of cross-infection.
- Recognize and manage critical situations or minor complications related to blood collection, such as hematomas and difficulty with venous access.
- Communicate effectively with patients, providing clear and reassuring explanations, to encourage collaboration and reduce anxiety in all age groups.
- Promote an ethical and responsible approach to the handling of biological material during collection, respecting the privacy, individuality, and dignity of the individual, and complying with current regulations.
- Apply sampling techniques correctly, respecting the type of analysis (chemical-physical, cultural, cytological, etc.) and adhering to the required guidelines and protocols.
- Ensure the integrity and quality of the sample collected through appropriate collection, storage and transport, avoiding contamination, alterations and deterioration.
- Use devices and instruments safely and effectively, with particular attention to the use of needles, test tubes, sterile containers, and personal protective equipment (PPE).
- Apply safety and biological risk prevention regulations, protecting yourself, the patient, and the work environment from exposure to potential hazards.
- Observe the principles of asepsis and antisepsis to reduce the risk of sample contamination and reduce the risk of cross-infection.
- Recognize and manage critical situations or minor complications related to blood collection, such as hematomas and difficulty with venous access.
- Communicate effectively with patients, providing clear and reassuring explanations, to encourage collaboration and reduce anxiety in all age groups.
- Promote an ethical and responsible approach to the handling of biological material during collection, respecting the privacy, individuality, and dignity of the individual, and complying with current regulations.
Course Prerequisites
Nobody
Teaching Methods
The course includes, in addition to the lecture, the viewing of videos, and the opportunity to manipulate the material covered in the lesson during these.
peer workshops are planned education supervised by the teacher/classroom tutor:
peer workshops are planned education supervised by the teacher/classroom tutor:
Assessment Methods
Final written test with 30 multiple-choice questions
Contents
- ANTISEPTICS AND DISINFECTANTS – A BRIEF GUIDE TO UNDERSTANDING AND USING THEM:
-Difference between antiseptics and disinfectants;
-Brief classification according to target organ, treated substrate;
-Factors influencing the activity of antiseptics and disinfectants;
-General principles for the correct use of antiseptics and disinfectants;
Precautions for use;
- Antiseptics and disinfectants useful in the collection of biological samples: ethyl and isopropyl alcohol, alcoholic chlorhexidine , chlorhexidine gluconate, non-soapy chlorhexidine gluconate, povidone iodine in alcoholic and aqueous solution, polyphenol,
- HAND HYGIENE DURING THE COLLECTION OF BIOLOGICAL SAMPLES:
-General principles;
-The 5 key moments for hand hygiene according to the WHO;
- Types of hand washing (purpose, times and methods) based on the biological sample to be collected;
- - Reasonable use of gloves: which types are best based on the type of sample to be collected.
- STERILE MATERIAL MANAGEMENT:
-What is sterility, purposes and indications;
-Brief presentation of the sterilization process;
- Sterilization methods: brief notes on the most commonly used materials.
-Types of packaging for sterile material: identify the expiration date, alterations that compromise sterility, and opening methods.
- -Handling of sterile material with NO-TOUCH technique
- COLLECTION OF BIOLOGICAL SAMPLES:
-Preliminary aspects: data collection and adjustment of the procedure based on the person's age; correct identification according to current regulations)
-Types of tests: culture, chemical-physical, cytological;
- Sputum collection : sample collection for culture examination, cytological examination, chemical-physical examination, search for Koch's bacillus (correct information and correct procedure, storage );
- Urine : sample collection for culture examination, cytological examination, chemical-physical examination, 24-hour urine collection, sample collection from a collection system with a bladder catheter or from a pediatric collecting bag or urine collection for multistix examination (correct information and correct procedure, storage );
- Stool : collection of occult blood sample, body culture, parasite screening, scotch tape test (correct information and correct procedure, storage );
- Collection of biological samples via swab : ear swab, nasal swab, throat swab, swab from infected wounds (correct information and posture, correct procedure, conservation);
- Saliva : (correct information, correct procedure, storage );
- Sperm (correct information, correct procedure, storage );
- Venous blood sample collection:
- Scope
- Preliminary aspects: data collection and adjustment of the procedure based on the person's age; correct identification according to current regulations)
- Anatomy of the peripheral venous system: basilic, cephalic, and metacarpal veins.
- How to identify the appropriate site: observation, palpation and inspection.
- Contraindications: edema, hematoma, plegia , phlebitis, surgical sutures, ipsilateral limb mastectomy.
- Characteristics of a blood collection needle;
- Preparation of the material and the person;
- Blood collection procedure with: classic syringe, vacutainer system and monovette system ;
- What to do if there is no blood flowing back into the collection system;
- Filling and storing test tubes: fundamental criteria;
- Considerations related to the patient's preparation in the pre-analysis phase : alcohol intake, particular foods, fasting, physical activity, posture, ongoing therapies;
- Evaluation of hemostasis: a fundamental element.
- Disposal of material.
- Sources of error during venous blood sampling;
-Difference between antiseptics and disinfectants;
-Brief classification according to target organ, treated substrate;
-Factors influencing the activity of antiseptics and disinfectants;
-General principles for the correct use of antiseptics and disinfectants;
Precautions for use;
- Antiseptics and disinfectants useful in the collection of biological samples: ethyl and isopropyl alcohol, alcoholic chlorhexidine , chlorhexidine gluconate, non-soapy chlorhexidine gluconate, povidone iodine in alcoholic and aqueous solution, polyphenol,
- HAND HYGIENE DURING THE COLLECTION OF BIOLOGICAL SAMPLES:
-General principles;
-The 5 key moments for hand hygiene according to the WHO;
- Types of hand washing (purpose, times and methods) based on the biological sample to be collected;
- - Reasonable use of gloves: which types are best based on the type of sample to be collected.
- STERILE MATERIAL MANAGEMENT:
-What is sterility, purposes and indications;
-Brief presentation of the sterilization process;
- Sterilization methods: brief notes on the most commonly used materials.
-Types of packaging for sterile material: identify the expiration date, alterations that compromise sterility, and opening methods.
- -Handling of sterile material with NO-TOUCH technique
- COLLECTION OF BIOLOGICAL SAMPLES:
-Preliminary aspects: data collection and adjustment of the procedure based on the person's age; correct identification according to current regulations)
-Types of tests: culture, chemical-physical, cytological;
- Sputum collection : sample collection for culture examination, cytological examination, chemical-physical examination, search for Koch's bacillus (correct information and correct procedure, storage );
- Urine : sample collection for culture examination, cytological examination, chemical-physical examination, 24-hour urine collection, sample collection from a collection system with a bladder catheter or from a pediatric collecting bag or urine collection for multistix examination (correct information and correct procedure, storage );
- Stool : collection of occult blood sample, body culture, parasite screening, scotch tape test (correct information and correct procedure, storage );
- Collection of biological samples via swab : ear swab, nasal swab, throat swab, swab from infected wounds (correct information and posture, correct procedure, conservation);
- Saliva : (correct information, correct procedure, storage );
- Sperm (correct information, correct procedure, storage );
- Venous blood sample collection:
- Scope
- Preliminary aspects: data collection and adjustment of the procedure based on the person's age; correct identification according to current regulations)
- Anatomy of the peripheral venous system: basilic, cephalic, and metacarpal veins.
- How to identify the appropriate site: observation, palpation and inspection.
- Contraindications: edema, hematoma, plegia , phlebitis, surgical sutures, ipsilateral limb mastectomy.
- Characteristics of a blood collection needle;
- Preparation of the material and the person;
- Blood collection procedure with: classic syringe, vacutainer system and monovette system ;
- What to do if there is no blood flowing back into the collection system;
- Filling and storing test tubes: fundamental criteria;
- Considerations related to the patient's preparation in the pre-analysis phase : alcohol intake, particular foods, fasting, physical activity, posture, ongoing therapies;
- Evaluation of hemostasis: a fundamental element.
- Disposal of material.
- Sources of error during venous blood sampling;
Course Language
Italian
More information
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Biomedical Laboratory techniques
Bachelor’s Degree
3 years
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