PASSI ALBERTO GIUSEPPE

Birthday: Cremona, 18.3.1962
EDUCATION
1995 Specialization
Specialization in Clinical Biochemistry cum laude, University of Pavia. Supervisor
prof. R.Moratti.
1992 Doctoral Degree
Doctoral Degree in Biochemistry, Department of Biochemistry, University of Pavia and Genova, Italy. Supervisor prof. G. Pallavicini.
1987 Degree
Degree in Medicine and Surgery cum laude, University of Pavia, Italy. Supervisor prof. A.A. Castellani.

ADDITIONAL EDUCATION
Graduate student, Medical School University of Munich (Germany) (1982 one semester)
Research Fellow, Chemical Morphology University of Manchester (UK) (1990 one month)
ACADEMIC APPOINTMENTS
(2001-present) Associate Professor, Department of Biomedical Experimental and Clinical Sciences, University of Insubria, Varese, School of Medicine
(1998-2001) Assistant Professor, Department of Biomedical Experimental and Clinical Sciences, University of Insubria, Varese, School of Medicine.
(1992-1998) Assistant Professor, Department of Biochemistry, University of Pavia, School of Medicine.
(1990-1998) Instructor of Biochemistry and Applied Biochemistry, University of Pavia School of Medicine.
(1998-present) Instructor of Biochemistry and Applied Biochemistry, University of Pavia School of Medicine.

EXPERIENCE
Research Scholar
Cleveland Clinic Foundation (2000-2001)
Postdoctoral fellow
Department of Biochemistry Universiy of Pavia (1991-1992)
Ph.D. student
Department of Biochemistry University of Pavia and Genova (1988-1991)

CLINICAL APPOINTMENTS
Emergency service (1989-1992)

SOCIETIES
Italian Biochemistry Society (1990-present)
Italian Connective Tissue Society (1990-present); member of the national board, treasurer of the society.
International Society of Matrix Biology (1998-present)
American Society for Matrix Biology (2003-present)
American Society of Biochemistry and Molecular Biology (2004-present)
AWARDS
National Fellowship University of Manchester (1990).
Alumnus of Collegio Ghislieri , Pavia, (1981-1987)

BOARD CERTIFICATION AND LICENSURE
1988, Italian Medical Association.

RESEARCH
1989-1991 Proteoglycan metabolism in bovine cornea during oxigen and lactate supply.
1991-1992 Proteoglycan metabolism in cell cultures: rat embryo cultures, study on adhesivity replicative subcultures.
1992-94. Proteoglycan metabolism in cell cultures obtained from Pseudoxanthoma patients.
1992-present. Extracellular matrix metabolism in cultures of human cells in senescence.
1993-present. Antioxidative effect of proteoglycan from different mammalian tissues.
1995-2001. Extracellular matrix in lung edema. Rabbit model.
2000-present. Hyaluronan metabolism in tissue and cell models.
2002-present. Use of stem cells in human transplant (bone and cartilage) and animal model (hearth)

RESEARCH FUNDING
1990-present. Italian University grants for scientific research.
1991-93 Italian CNR grants of Italian University Funds (MURST) “Progetto finalizzato invecchiamento”.
1993-95. Lombardy Regional Funds for scientific Research.
1996. Italian CNR grant (Edema project).
1997-1998 Italian National Project on “Proteoglycan metabolism in normal and pathologic conditions”
1998. Italian CNR grant on Lung proteolgycan.
2000-2002: Excellence National project from Italian Minister of Research: MMP and matrix
2002-2004: Excellence National project from Italian Minister of Research: Hyaluronan in cancer
2003-2004: Excellence National project from Insubria Atenaeum: Plasma MMPs and collagen peptides
2004-2005: Biotechnology National Fund: Cloning of UDPglucose DH from X.Laevis and his role in embriogenesis and his biochemical characterization in eucariotic cells.
Reviewer of several scietific journals, including Journ of Biological Chemistry, Analytical Biochemistry, Biochemie, International Journa of Cancer, Archives of Dermatology ect
Member of several University Commitees, including Evaluation Board, International Activity Board, Research Fund Board.
2012-2014 IRSES, EU funds FP7: progetto “INFLAMA” n° 2475126, Project leader and European responsible.
2015-2017 RISE, EU funds Horizon 2020: progetto “Glycanc” participant



INTERNATIONAL MEETINGS
Prof. Passi anttended several national and international meetings presenting talks and acting as chairman. Follow the list of most important scientific meetings:
1. XIIth FECTS MEETING, Bialistok (Poland), 9-13 July 1990; (selected talk)
2. A.S.G.B.I., B.S.C.B., Joint Meeting, Manchester (UK) 3-6 April 1990;
3. Joint Symposia Italian Biochemical Society, Dutch Biochemical Society and Union of Biochemical Societies of Yugoslavia, Bari,(Italy) 1990, (selected talk );
4. Conference on Heritable connective Tissue Disorders, Siviglia (Spain) 7-9 March 1991 (selected talk);
5. 639th Biochemical Society meeting, Manchester, (UK) July 1991;
6. Biochemistry of the Eye,Manchester (UK) July 1991;
7. XIIIth FECTS Meeting, Davos (Switzerland), July 1992;
8. 8th Harden Discussion, Manchester (UK) March 1993; (invited talk);
9. British Connective Tissue Society Meeting, Manchester (UK) March 1993;
10. XIVth FECTS Meeting, Lyon (France), August 1994;
11. Lipoprotein Oxidation and Atherosclerosis, Pavia, (Italy) September 1994;
12. International Conference on Cell Death in Human Pathology, Lecce, (Italy) June 1995; (selected talk);
13. 1st European Congress of the European Physiological Societies. Maastricht (NL) September 1995; (selected talk)
14. Federation of Experimental Biology Societies Meeting. Washington (USA) April 1996.
15. FEBS Meting, Barcellona (Spain) July 1996;
16. XVth FECTS Meeting , Munich (Germany) August 1996.
17. British Connective Tissue Society Meeting, Lancaster (UK) April 1997 (invited speaker).
18. XXXIII International Congress of Physiological Sciences. St. Petersburg (Russia) June 1997 (invited speaker)
19. American gastroenterological Association and American Association for the study of liver diseases: annual meeting, Washington (USA) May 1997.
20. Ecosep1st course and Greek connective society meeting. Patras (Greece) 1998 (invited speaker).
21. American Respiratory Physiology Society Congress, Aspen (USA) 1998 .
22. XVI FECTS Meeting Uppsala (Svezia) 1998.
23. 8th Conference of European Orthopaedic Research Society, Amsterdam (Olanda),1998.
24. American Respiratory Physiology Society, Congress, Jasper (Canada) 1999.
25. FASEB: Experimental Biology Meeting. Washington (USA) 1999.
26. XVII FECTS Meeting Patras (Greece) 2000.
27. FASEB: Experimental Biology Meeting. San Diego (USA) 2000.
28. 4th International Symposium on Molecular Medicine, Creta,(Greece) 2001.
29. American Investigative Dermatology Meeting, Washington (USA), 2001
30. XVIII Federation of European Connective Tissue Societies (FECTS) Meeting, Brighton (UK) 2002 (invited speaker)
31. American Investigative Dermatology Meeting, Los Angeles (USA) 2002
32. Harden Discussion on Biopathology of Proteoglycans, Parma (Italy) 2003 (invited speaker)
33. Hyaluronan 2003, Cleveland (USA) 2003 (invited speaker)
34. XIX FECTS Meeting, Giardini Naxos (Italy) 2004 (selected talk)
35. Gordon Conference on Proteoglycans, Hartford (USA) 2004
36. Proteoglycan and cell signalling, Harden Discussion, Runo, Sweden, 2005
37. XVIII Glyco Conference, Firenze, 2005 (selected talk)
38. Gordon Conference on Proteoglycans, Andover NH USA, 2006
39. XX FECTS Meeting, Oulu (Finland) 2006
40. Hyaluronan 2007, Charleston (SC-USA) (selected talk)
41. FEBS Selected Course, Matrix Pathobiology, Patras (Greece) May 2007 (invited speaker)
42. Carla Workshop of Fabre Laboratories, Soreze (France) 2007 (invited speaker)
43. 4th Finnish Glycoscience Symposium, Rautavaara (Finland) 2007 (invited speaker)
44. International Biochemistry Societies Congress Athens (Greece) 2008 (invited speaker)
45. Gordon Conference on Proteoglycans Andover NH USA(invited speaker) 2008
46. Hellenic National Biochemistry Meeting Patras (Greece) 2009 (invited speaker)
47. FEBS Course on Matrix Pathobiology, invited speaker Patras (Greece) 2009
48. Hyaluronan 2010, Kyoto (Japan) June 2010 (invited speaker)
49. Gordon Conference on Proteoglycans, Andover NH USA, 2010
50. 8th Finnish Glycoscience Symposium, Kuopio (Finland) 2010 (invited speaker)
51. Experimental Biology Meeting EB 2011, Washington (USA)
52. FEBS Selected Course, Matrix Pathobiology, Spetses (Greece) September 2011 (invited speaker)
53. Experimental Biology Meeting EB 2012, San Diego (USA)
54. Brazilian Society of Biochemistry meeting –SBBq Foz Uguazù (Brazil) 2012 (Invited speaker)
55. EB 2013 in Glycobiology workshop Boston (MA USA) (selected speaker)
56. Hyaluronan 2013 Oklahoma City (OK – USA) (invited speaker)
57. Proteoglycans 2013 Intl. Meeting (Frankfurt – Germany) (invited speaker)
58. FEBS Selected Course, Matrix Pathobiology, Kos (Greece) 2013 (invited speaker)
59. Symposium “Targeting Hyaluronan in Pathophysiology and Disease” Duesseldorf (Germany) 2014 (invited speaker)
60. Gordon Conference on Proteoglycans, Andover NH USA, 2014 (invited speaker)
61. Hyaluronan 2015, Florence 2015 (organizer)
62. 9th International Conference on Proteoglycans and 10th Pan-Pacific Connective Tissue Societies Symposium, Seoul (South Korea) 2015
63. XXIII Glyko meeting Split (Croatia) 2015 (invited speaker)
64. 5th FEBS Advanced Lecture Course Matrix Pathobiology, Signaling and Molecular Targets
65. Rhodes (Greece) 2015, (invited speaker)
66. 2nd MBE European Matrix biology Meeting Athens (Greece) 2016 (Invited speaker)
67. Gordon Conference on Proteoglycans Andover NH (USA) 2016 (selected speaker)

BIBLIOGRAPHY
1. Balduini,C.; De Luca, G.; Passi,A.; Rindi,S.; Salvini, R.; Scott, J.E. “Differential effect of oxygen tension and lactate concentration on keratansulphate and hexuronate-containing glycosaminoglycan production in cornea-” (1990) Journal of Anatomy, vol.1 pag 200. (I.F. 2,39)
2. Balduini,C.; De Luca, G.; Passi,A.; Rindi,S.; Salvini, R.; Scott, J.E. “Differential effect of oxygen supply and lactate concentration on keratansulphate and glycosaminoglycuronan production in bovine cornea.” (1991) Biochem.Soc.Trans.;vol 19:354. (I.F. 2,267)
3. Balduini, C.; De Luca, G.; Passi, A.; Rindi, S.; Salvini, R.; Scott, J.E. “Effect of oxygen tension and lactate concentration on keratansulphate and chondroitinsulphate biosynthesis in bovine cornea.” (1992) Biochim.Bioph.Acta (General subjects), vol.1115,pag.187-191. (I.F.3,369)
4. De Luca, G.; Passi,A. “Involvement of proteoglycan metabolism regulation in aging and atherosclerosis.” (1992) In "Biology of Aging"; Zwilling & Balduini ed.; Springer Verlag, Heidelberg, pag 106-118.
5. Pippia, P.; Formato, M.; Sciola, L.; Tilloca, G.; Bardoni, A.; Pallavicini, G.; Passi, A.; Rindi, S.; Salvini, R.; De Luca, G. “Modifications of adhesion properties and proteoglycan structure in rat fibroblast cultures with increasing passages.” (1992) Cell Biochemistry and Funct.,vol.10,pag.217-224. (I.F. 1,179)
6. Passi, A.; Balduini, C.; De Luca, G.; Pallavicini, G.; Scott, J.E. “Dermatansulphate biosynthesis: NAD influences iduronate formation.” (1993) in “ The Dermatansulphate and Dermatansulphate proteoglycans”; Scott J.E. ed.; cap.9, pag 139-145;, Portland Press, London.
7. Passi, A.; Albertini, R.; Bardoni, A.; Rindi, S.; Salvini, R.; Pallavicini, G.; De Luca, G. “Modifications of proteoglycans produced by human skin fibroblast cultures during replicative senescence.” (1993) Cell Biochemistry and Funct., vol.11, pag 263-269. (I.F. 1,179)
8. Bardoni A., Salvini R., Pallavicini G., Passi A., Rindi S., Quacci D., Dall'Orbo C., De Luca G. “Collagenase extractable proteoglycans from lesion-free areas of human aorta.” (1994) It. J. of Biochem., vol.43 n.1, pag 1-23. (I.F.0,000)
9. Albertini, R.; Rindi, S.; Passi, A.; Palladini, G.; Pallavicini,G.; De Luca,G. “The effect of heparin on Cu++-mediated oxidation of human low density lipoproteins”. (1995) FEBS Letters 377, 240-242. (I.F. 3,843)
10. Passi, A.; Albertini, R.; Baccarani Contri, M.; De Luca, G.; De Paepe, A.; Pallavicini, G.; Pasquali Ronchetti, I.; Tiozzo, R. “ Proteoglycan alterations in skin fibroblast cultures from patients affected with pseudoxanthoma elasticum”. (1996) Cell Biochemistry and Funct. 14, 111-120. (I.F. 1,179)
11. Cristallo A, Passi A, Bruseghini L, Cereda PM Pseudomonas aeruginosa proteases: Purification procedures for an enzymatic standard (1996) Microbiologica 19 (3): 221-226. (I.F. 0,431)
12. Albertini R., Rindi S., Passi A., Bardoni A., Salvini R., Pallavicini G., De Luca G. “The effect of cornea proteoglycans on liposome peroxidation” (1996) Arch.Biochem.Biophys. 327, 209-214. (I.F. 2,647)
13. Albertini R., Rindi S., Passi A, Pallavicini G., De Luca G. “Heparin protectionagainst Fe2+ and Cu2+ mediated oxidation of liposomes” (1996) FEBS Letters 383, 155-158. (I.F. 3,843)
14. Negrini, D.; Passi, A.; De Luca, G.; Miserocchi, G. “Pulmonary interstitial pressure and proteoglycans during development of pulmonary edema” (1996) Am. J. Physiol. 270 (Heart Circ. Physiol. 39), H2000-2007. (I.F. 3,539)
15. Albertini R., Rindi S., Passi A, Pallavicini G., De Luca G. “Proective effect of proteoglycans and sulphated glycosaminoglycans on lipid peroxidation”. (1996) In Biology and Physiopathology of the extracellular matrix ” Balduini, Cherubino and De Luca eds. La Goliardica Pavese pag.49-60.
16. Albertini, R.; Ramos, P.; Giessauf, A.; Passi, A.; De Luca, G.; Hesterbauer, H. “Chondroitin-4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation”. (1997) FEBS Letters 403, 154-158. (I.F. 3,843)
17. Passi, A.; Albertini, R.; Campagnari, F.and De Luca G. “Modifications of proteoglycans secreted into the growth medium by young and senescent human skin fibroblasts”. (1997) FEBS Letters 402, 286-290. (I.F. 3,843)
18. Passi, A.; Albertini, R.; Campagnari, F.and De Luca G. “Modifications of proteoglycans extracted from monolayer cultures of young and senescent fibroblasts”. (1997) FEBS Letters 420, 175-178. (I.F. 3,843)
19. Negrini, D.; Passi, A.; De Luca, G.; Miserocchi, G. “Proteoglycan involvement during development of lesional pulmonary edema” (1998) Am. J. Physiol. vol. 274 (2 Pt 1),L203-211. (I.F.4,051)
20. Negrini, D.; Passi, A.; De Luca, G.; Miserocchi, G. “Role of matrix macromolecules in controlling lung fluid balance: transition from a dry tissue to edema.” (1998) in “Cellular and Molecular Mechanims and Clinical Management” S. Matalon ed.,Plenum Press pag. 292-304.
21. Albertini, R., Abuja, P., Passi, A., De Luca, G. “Chondroitin-4-sulphate, but not chondroitin-6-sulphate, inhibits copper-induced oxidation of LDL and HDL.” (1998) In Proceedings of the european training program in microseparation techniques (Ecosep1) pag. 415-425.
22. Passi, A.; Albertini, De Luca G.; Miserocchi, G; Negrini, D. “Proteoglycans in Pulmonary edema” (1998) In Proceedings of the european training program in microseparation techniques (Ecosep1) pag. 426-440.
23. Passi, A., Negrini, D.; Albertini, R.; De Luca, G.; Miserocchi, G.:” Involvement of lung interstitial proteoglycans in development of hydraulic- and elastase-induced edema.” (1998) Am J Physiol. Vol.275 (3Pt.1):L631-L635. (I.F. 4,051)
24. Passi, A., Negrini, D.; Albertini, R.; De Luca, G.; Miserocchi, G.:” The sensitivity of versican from rabbit lung to gelatinase A (MMP-2) and B (MMP-9) and its involvement in the development of hydraulic lung edema. “ (1999) FEBS Lett. 30; 456 (1) :93-6. (I.F. 3,843)
25. Miserocchi, G., Passi, A., Albertini, R., Negrini, D., De Luca, G. “ Interstitial pressure and proteoglycan degradation in hydraulic- and elastase-induced lung edema.” (1999) Chest.;116:31. (I.F. 3,118)
26. Albertini, R., De Luca, G., Passi, A., Moratti, R., Abuja, P.” Chondroitin-4-sulfate protects high-density lipoprotein against copper-dependent oxidation. “ (1999) Arch Biochem Biophys. 1;365(1):143-9. (I.F. 2,647)
27. Albertini R, De Luca G, Palladini G, Passi A, Melzi d'Eril GV, Abuja PM “ Mechanistic aspects of the relationship between low-level chemiluminescence and lipid peroxides in oxidation of low-density lipoprotein. “ (1999) FEBS Lett.;459(1):47-50. (I.F. 3,843)
28. Albertini R. Passi A. Abuja PM. De Luca G. The effect of glycosaminoglycans and proteoglycans on lipid peroxidation (Review). (2000) International Journal of Molecular Medicine. 6(2):129-136. (I.F. 3,190)
29. Negrini D., Passi A., Bertin K., Bosi F., Wiig H. “ Isolation of pulmonary interstitial fluid in rabbits by a modified wick technique”. (2001) Am J Physiol. Lung Cellular & Molecular Physiology. 280(5):L1057-65. (I.F. 4,051)
30. Miserocchi G., Negrini D., Passi A. De Luca G “ Development of lung edema: interstitial fluid dynamics and molecular structure” (2001) News Physiol Sci. 16:66-71. (I.F. 3,306)
31. Miserocchi G., Passi A.,Negrini D., Del Fabbro M., De Luca G. “Pulmonary interstitial pressure and tissue matrix structure in acute hypoxia” (2001) Am J Physiol. Lung Cellular & Molecular Physiology. 280(5):L881-7. (I.F. 4,051)
32. Leoni V., Albertini R., Passi A., Abuja PM., Borroni P., Melzi d’Eril G., De Luca G. “ Glucose accelerates copper-and ceruplasmin-induced oxidation of low density lipoprotein and whole serum”. (2002) Free Rad. Res; 36(5); pp.521-529. (I.F. 2,744)
33. Negrini D., Passi A., De Luca G. and Miserocchi G. “Matrix proteoglycans in Development of Pulmonary Edema” (2002) in Proteoglycans in Lung Disease, Garg H.G., Roughley PJ and Hales C.A. eds; Marcel Dekker Inc; New York-Basel; pagg.143-168.
34. Sciannamblo MT, Chigorno V, Passi A, Valaperta R, Zucchi I and Sandro Sonnino “Changes of the gangliosides pattern and content in human fibroblasts by high density population subculture progression” (2002) Glycoconjugate Journal, 19, 181-186. (I.F. 0,737)
35. Vigetti D., Viola M., Gornati R., Ori M., Nardi I., Passi A., De Luca G., Bernardini G. “Molecular cloning, genomic organization and developmental expression of the Xenopus laevis hyaluronan synthase 3”. (2003) In Matrix biology, 22, pagg 511-517. (I.F. 4,104)
36. Karousou E., Porta G., De Luca G. and Passi A. Analysis of fluorophore-labelled hyaluronan and chondroitin sulfate disaccharides in biological samples (2003) Journal of Pharmaceutical and Biomedical Analysis vol 34/4, 791-795. (I.F. 1,509)
37. Raio L., Ghezzi F., Cromi A., Cereda E., Passi A. Sonographic Morphology and Hyaluronan Content of Umbilical Cords of Healthy and Down Syndrome Fetuses (2004) Early Gestation Earl Human Dev. vol.77, 1-12 (I.F. 1,000)
38. Passi A., Sadeghi P., Kawamura H., Anand S., Sato N., White L.E., Hascall V.C., Maytin E.V. hyaluronan suppresses late markers of epidermal differentiation in organotypic cultures of rat keratinocytes (2004) Experimental Cell Research 296/2, 123-134 (I.F. 4,007)
39. Karousou E.G., Militsopoulou M., Porta G., De Luca G., Hascall VC, Passi A. Polyacrylamide gel electrophoresis of fluorophore-labeled hyaluronan and chondroitin sulfate disaccharides. Application to the analysis in cells and tissues. (2004) Electrophoresis vol. Volume 25, Issue 17 , Pages 2919 - 2925 (I.F. 3,743)
40. Passi Alberto, Raio Luigi, Cereda Emanuele, Ghezzi Fabio, Pallotti Francesco, Porta Giovanni, Cromi Antonella, Bolis Pierfrancesco, De Luca Giancarlo. Hyaluronan content of umbilical cords of healthy and Down Syndrome fetuses. (2004) in Hyaluronan Its Structure, Metabolism, Biological Activities and Therapeutic Applications (E. A.Balasz and VC Hascall eds.), Vol 1, 2005, Publishers: Winmar Enterprises.
41. Vigetti D., Viola M., Cereda E., Gornati R., Karousou E., Ori M., Nardi I., Bernardini G., Passi A., De Luca G. Hyaluronan in Xenopus laevis development. in Hyaluronan Its Structure, Metabolism, Biological Activities and Therapeutic Applications (E. A.Balasz and VC Hascall eds.), Vol 1, 2005, Publishers: Winmar Enterprises. www.matrixbiologyinstitute.org/ha03/ch2/index.htm
42. Daniela Quaglino; Luigi Sartor; Spiridione Garbisa; Federica Boraldi; Antonietta Croce; Alberto Passi; Giancarlo De Luca; Roberta Tiozzo; Ivonne Pasquali Ronchetti. Dermal fibroblasts from Pseudoxanthoma elasticum patients have raised MMP-2 degradative potential. (2005) Biochim. Biophys. Acta - Molecular Basis of Disease, 1741 (1-2): 42-47. (I.F. 3,046)
43. Luigi Raio, Antonella Cromi, Fabio Ghezzi, Alberto Passi, Eugenia Karousou, Manuela Viola, Davide Vigetti , Giancarlo De Luca and Pierfrancesco Bolis. Hyaluronan content of Wharton´s jelly in healthy and Down syndrome fetuses. (2005) Matrix biology, vol 24 (2) : 166-74. ( IF 4,104)
44. Evgenia G. Karousou, Manuela Viola, Anna Genasetti, Davide Vigetti, Giancarlo De Luca, Nikos K. Karamanos, Alberto Passi. Application of polyacrylamide gel electrophoresis of fluorophore labeled-saccharides for analysis of hyaluronan and chondroitin sulfate in human and animal tissues and cell cultures. (2005) Biomedical Chromatography vol 19(10):761-5. (IF 1,069)
45. Antoine Malek, N.E Bersinger, S. Di Santo, M.D. Mueller, R. Sanger, H. Schneider, F. Grezzi, E. Karousou, A. Passi, G. De Luca, L. Raio. C-Reactive Protein production in term human placental tissue. (2006) Placenta 26 (6-7) 619-25 (IF 2,683)
46. Negrini D, Tenstad O, Passi A, Wiig H. Differentiale degradation of matrix proteoglycans and edema development in rabbit. (2006) Am J Physiol Lung Cell Mol Physiol. 290 (3): L470-L477 (IF 4,051)
47. Mattarucchi E, Marsoni M, Binelli G, Passi A, Lo Curto F, Pasquali F, Porta G. Different real time PCR approaches for the fine quantification of SNP's alleles in DNA pools: assays development, characterization and pre-validation. (2005) J Biochem Mol Biol. 2005 Sep 30;38(5):555-62. (IF 1,53)
48. Viola M,Karousou EG, Vigetti D, Genasetti A, Pallotti F,Guidetti GF, Tira E, De Luca G, Passi A Decorin from different bovine tissues: study of glycosaminoglycan chain by PAGEFS. (2006) Journal of Pharmaceutical and Biomedical Analysis 41(1) 36-42 (I.F. 1,509)
49. Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G and Passi A Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian xenopus laevis and its involvement in hyaluronan synthesis (2006) J. Biol. Chem, 281 8254-8263 (I.F. 6,35)
50. Uccella, S., Cerutti, R., Vigetti, D., Furlan, D., Oldrini, R., Carnevali, I., Pelosi, G., La Rosa, S., Passi, A., Capella, C. (2006). Histidine decarboxylase, dopa decarboxylase and vesicular monoamine transporter 2 expression in neuroendocrine tumors: immunohistochemical study and gene expression analysis. Journal of Histochemistry and Cytochemistry in press (I.F. 2.513).
51. Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Pallotti F, De Luca G and Passi A. Matrix metalloproteinase 2 and tissue inhibitors of metalloproteinases regulate human aortic smooth muscle cell migration during in vitro aging. (2006) FASEB J. 20, 1118-1130 (2006). (I.F. 6.853)
52. Mattarucchi E, Marsoni M, Passi A, Lo Curto F, Pasquali F, Porta G. Establishment and study of different real-time polymerase chain reaction assays for the quantification of cells with deletions of chromosome 7. J Mol Diagn. 2006 May;8(2):218-24. (I.F. 2.716)
53. Negrini, D., Passi A. Interstitial matrix and transendothelial fluxes in normal lung.
Respir Physiol Neurobiol. 2007 2007 Apr 8; [Epub ahead of print]
PMID: 17499562
54. Moriondo A., Pelosi P., Passi A., Viola M., Marcozzi C., Severgnini P., Ottani V., Quaranta M., Negrini D. Proteoglycans fragmentation and respiratory mechanics in mechanically ventilated healthy rats. J Appl Physiol. 2007 Sep;103(3):747-56. (I.F. 3,178)
55. Pelosi P, Rocco PR, Negrini D, Passi A. The extracellular matrix of the lung and its role in edema formation. An Acad Bras Cienc. 2007 Jun;79(2):285-97. PMID: 17625682 [PubMed - in process]
56. Genasetti A, Valentino ML, Carelli V, Vigetti D, Viola M, Karousou EG, Melzi d'Eril GV, De Luca G, Passi A, Pallotti F. Assessing Heteroplasmic Load in Leber's Hereditary Optic Neuropathy Mutation 3460G->A/MT-ND1 with A Real-Time PCR Quantitative Approach. J Mol Diagn. 2007 Sep;9(4):538-45. Epub 2007 Jul 25. PMID: 17652639 [PubMed - in process] (IF 2,716)
57. Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A. Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2008 Feb 15;283(7):4448-58.
58. Negrini D, Passi A, Moriondo A. The role of proteoglycans in pulmonary edema development. Intensive Care Med. 2008 Apr;34(4):610-8.
59. Karousou E, Ronga M, Vigetti D, Passi A, Maffulli N. Collagens, proteoglycans, MMP-2, MMP-9 and TIMPs in human achilles tendon rupture. Clin Orthop Relat Res. 2008 Jul;466(7):1577-82.
60. Viola M, Vigetti D, Karousou E, Bartolini B, Genasetti A, Rizzi M, Clerici M, Pallotti F, De Luca G, Passi A. New electrophoretic and chromatographic techniques for analysis of heparin and heparan sulfate. Electrophoresis. 2008 Aug;29(15):3168-74.
61. Viola M, Vigetti D, Genasetti A, Rizzi M, Karousou E, Moretto P, Clerici M, Bartolini B, Pallotti F, De Luca G, Passi A. Molecular control of the hyaluronan biosynthesis. Connect Tissue Res. 2008;49(3):111-4.
62. Genasetti A, Vigetti D, Viola M, Karousou E, Moretto P, Rizzi M, Bartolini B, Clerici M, Pallotti F, De Luca G, Passi A. Hyaluronan and human endothelial cell behavior. Connect Tissue Res. 2008;49(3):120-3.
63. Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Clerici M, Bartolini B, Pallotti F, De Luca G, Passi A. Aortic smooth muscle cells migration and the role of metalloproteinases and hyaluronan. Connect Tissue Res. 2008;49(3):189-92.
64. Pirrone F, Mazzola SM, Pastore C, Paltrinieri S, Sironi G, Riccaboni P, Viola M, Passi A, Clement MG, Albertini M. Activated protein C protection from lung inflammation in endotoxin-induced injury. Exp Biol Med (Maywood). 2008 Nov;233(11):1462-8.
65. Vigetti D, Andrini O, Clerici M, Negrini D, Passi A, Moriondo A. Chondroitin sulfates act as extracellular gating modifiers on voltage-dependent ion channels. Cell Physiol Biochem. 2008;22(1-4):137-46.
66. Vigetti D, Viola M, Karousou E, Genasetti A, Rizzi M, Clerici M, Bartolini B, Moretto P, De Luca G, Passi A. Vascular pathology and the role of hyaluronan. ScientificWorldJournal. 2008 Nov 2;8:1116-8.
67. Cordo-Russo R, Garcia MG, Barrientos G, Orsal AS, Viola M, Moschansky P, Ringel F, Passi A, Alaniz L, Hajos S, Blois SM. Murine abortion is associated with enhanced hyaluronan expression and abnormal localization at the fetomaternal interface. Placenta. 2009 Jan;30(1):88-95. Epub 2008 Dec 6. PubMed PMID:19059644.
68. Vigetti D, Rizzi M, Viola M, Karousou E, Genasetti A, Clerici M, Bartolini B, Hascall VC, De Luca G, Passi A. The effects of 4-methylumbelliferone on hyaluronan synthesis, MMP2 activity, proliferation, and motility of human aortic smooth muscle cells. Glycobiology. 2009 May;19(5):537-46. Epub 2009 Feb 24.PubMed PMID: 19240269.
69. Tajana MS, Murena L, Valli F, Passi A, Grassi FA. Correlations between biochemical markers in the synovial fluid and severity of rotator cuff disease. Chir Organi Mov. 2009 Apr;93 Suppl 1:S41-8. PubMed PMID: 19711169.
70. Vigetti D, Genasetti A, Karousou E, Viola M, Clerici M, Bartolini B, Moretto P, De Luca G, Hascall VC, Passi A. Modulation of hyaluronan synthase activity in cellular membrane fractions. J Biol Chem. 2009 Oct 30;284(44):30684-94. Epub 2009 Sep 8. PubMed PMID: 19737932; PubMed Central PMCID: PMC2781622.
71. Karousou E, Ronga M, Vigetti D, Barcolli D, Passi A, Maffulli N. Molecular interactions in extracellular matrix of tendon. Front Biosci (Elite Ed). 2010 Jan 1;2:1-12. PubMed PMID: 20036847
72 : Gualeni B, Facchini M, De Leonardis F, Tenni R, Cetta G, Viola M, Passi A, Superti-Furga A, Forlino A, Rossi A. Defective proteoglycan sulfation of the growth plate zones causes reduced chondrocyte proliferation via an altered Indian hedgehog signalling. Matrix Biol. 2010 Jul;29(6):453-60.
73: Karousou E, Kamiryo M, Skandalis SS, Ruusala A, Asteriou T, Passi A, Yamashita H, Hellman U, Heldin CH, Heldin P. The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination. J Biol Chem. 2010 Jul 30;285(31):23647-54
74: Vigetti D, Genasetti A, Karousou E, Viola M, Moretto P, Clerici M, Deleonibus S, De Luca G, Hascall VC, Passi A. Proinflammatory cytokines induce hyaluronan synthesis and monocyte adhesion in human endothelial cells through hyaluronan synthase 2 (HAS2) and the nuclear factor-kappaB (NF-kappaB) pathway. J Biol Chem.2010 Aug 6;285(32):24639-45.
75: Karangelis DE, Kanakis I, Asimakopoulou AP, Karousou E, Passi A, Theocharis AD, Triposkiadis F, Tsilimingas NB, Karamanos NK. Glycosaminoglycans as key molecules in atherosclerosis: the role of versican and hyaluronan. Curr Med Chem. 2010;17(33):4018-26.
76: Acquati F, Bertilaccio S, Grimaldi A, Monti L, Cinquetti R, Bonetti P, Lualdi M, Vidalino L, Fabbri M, Sacco MG, van Rooijen N, Campomenosi P, Vigetti D, Passi A, Riva C, Capella C, Sanvito F, Doglioni C, Gribaldo L, Macchi P, Sica A, Noonan DM, Ghia P, Taramelli R. Microenvironmental control of malignancy exerted by RNASET2, a widely conserved extracellular RNase. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1104-9.
77: Vigetti D, Clerici M, Deleonibus S, Karousou E, Viola M, Moretto P, Heldin P, Hascall VC, De Luca G, Passi A. Hyaluronan synthesis is inhibited by adenosine monophosphate-activated protein kinase through the regulation of HAS2 activity in human aortic smooth muscle cells. J Biol Chem. 2011 Mar 11;286(10):7917-24.
78: Tammi RH, Passi AG, Rilla K, Karousou E, Vigetti D, Makkonen K, Tammi MI.Transcriptional and post-translational regulation of hyaluronan synthesis. FEBS J. 2011 May;278(9):1419-28.
79: Merrilees MJ, Beaumont BW, Braun KR, Thomas AC, Kang I, Hinek A, Passi A, Wight TN. Neointima formed by arterial smooth muscle cells expressing versican variant V3 is resistant to lipid and macrophage accumulation. Arterioscler Thromb Vasc Biol. 2011 Jun;31(6):1309-16.
80: Moriondo A, Marcozzi C, Bianchin F, Passi A, Boschetti F, Lattanzio S, Severgnini P, Pelosi P, Negrini D. Impact of respiratory pattern on lung mechanics and interstitial proteoglycans in spontaneously breathing anaesthetized healthy rats. Acta Physiol (Oxf). 2011 Oct;203(2):331-41.
81: Vigetti D, Rizzi M, Moretto P, Deleonibus S, Dreyfuss JM, Karousou E, Viola M, Clerici M, Hascall VC, Ramoni MF, De Luca G, Passi A. Glycosaminoglycans and glucose prevent apoptosis in 4-methylumbelliferone-treated human aortic smooth muscle cells. J Biol Chem. 2011 Oct 7;286(40):34497-503.
82: Piccioni F, Malvicini M, Garcia MG, Rodriguez A, Atorrasagasti C, Kippes N, Piedra Buena IT, Rizzo MM, Bayo J, Aquino J, Viola M, Passi A, Alaniz L, Mazzolini G. Antitumor effects of hyaluronic acid inhibitor 4-methylumbelliferone in an orthotopic hepatocellular carcinoma model in mice. Glycobiology. 2012 Mar;22(3):400-10.
83: Moriondo A, Marcozzi C, Bianchin F, Reguzzoni M, Severgnini P, Protasoni M, Raspanti M, Passi A, Pelosi P, Negrini D. Impact of mechanical ventilation and fluid load on pulmonary glycosaminoglycans. Respir Physiol Neurobiol. 2012 May 31;181(3):308-20.
84: Vigetti D, Deleonibus S, Moretto P, Karousou E, Viola M, Bartolini B, Hascall VC, Tammi M, De Luca G, Passi A. Role of UDP-N-acetylglucosamine (GlcNAc) and O-GlcNAcylation of hyaluronan synthase 2 in the control of chondroitin sulfate and hyaluronan synthesis. J Biol Chem. 2012 Oct 12;287(42):35544-55.
85: Karousou E, Stachtea X, Moretto P, Viola M, Vigetti D, D'Angelo ML, Raio L, Ghezzi F, Pallotti F, De Luca G, Karamanos NK, Passi A. New insights into the pathobiology of Down syndrome--hyaluronan synthase-2 overexpression is regulated by collagen VI α2 chain. FEBS J. 2013 May;280(10):2418-30.
86: Motolese A, Vignati F, Brambilla R, Cerati M, Passi A. Interaction between a regenerative matrix and wound bed in nonhealing ulcers: results with 16 cases. Biomed Res Int. 2013;2013:849321. d
87: Viola M, Bartolini B, Vigetti D, Karousou E, Moretto P, Deleonibus S, Sawamura T, Wight TN, Hascall VC, De Luca G, Passi A. Oxidized low density lipoprotein (LDL) affects hyaluronan synthesis in human aortic smooth muscle cells. J Biol Chem. 2013 Oct 11;288(41):29595-603.
88: Vigetti D, Viola M, Karousou E, De Luca G, Passi A. Metabolic control of hyaluronan synthases. Matrix Biol. 2014 Apr;35:8-13.
89: Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW, Hart GW. The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc. Matrix Biol. 2014 Apr;35:14-7.
90: Vigetti D, Karousou E, Viola M, Deleonibus S, De Luca G, Passi A. Hyaluronan: biosynthesis and signaling. Biochim Biophys Acta. 2014 Aug;1840(8):2452-9.
91: Gialeli Ch, Viola M, Barbouri D, Kletsas D, Passi A, Karamanos NK. Dynamic interplay between breast cancer cells and normal endothelium mediates the expression of matrix macromolecules, proteasome activity and functional properties of endothelial cells. Biochim Biophys Acta. 2014 Aug;1840(8):2549-59.
92: Abbate GM, Borghi D, Passi A, Levrini L. Correlation between unstimulated salivary flow, pH and streptococcus mutans, analysed with real time PCR, in caries-free and caries-active children. Eur J Paediatr Dent. 2014 Mar;15(1):51-4.
93: Karousou E, Asimakopoulou A, Monti L, Zafeiropoulou V, Afratis N, Gartaganis P, Rossi A, Passi A, Karamanos NK. FACE analysis as a fast and reliable methodology to monitor the sulfation and total amount of chondroitin sulfate in biological samples of clinical importance. Molecules. 2014 Jun 12;19(6):7959-80.
94: Vigetti D, Viola M, Karousou E, Deleonibus S, Karamanou K, De Luca G, Passi A. Epigenetics in extracellular matrix remodeling and hyaluronan metabolism. FEBS J. 2014 Nov;281(22):4980-92.
95: Vigetti D, Passi A. Hyaluronan synthases posttranslational regulation in cancer. Adv Cancer Res. 2014;123:95-119.
96: Karousou E, D'Angelo ML, Kouvidi K, Vigetti D, Viola M, Nikitovic D, De Luca G, Passi A. Collagen VI and hyaluronan: the common role in breast cancer. Biomed Res Int. 2014;2014:606458.
97: Nikitovic D, Kouvidi K, Voudouri K, Berdiaki A, Karousou E, Passi A, Tzanakakis GN. The motile breast cancer phenotype roles of proteoglycans/glycosaminoglycans. Biomed Res Int. 2014;2014:124321.
98: Vigetti D, Deleonibus S, Moretto P, Bowen T, Fischer JW, Grandoch M,Oberhuber A, Love DC, Hanover JA, Cinquetti R, Karousou E, Viola M, D'Angelo ML, Hascall VC, De Luca G, Passi A. Natural antisense transcript for hyaluronan
synthase 2 (HAS2-AS1) induces transcription of HAS2 via protein O-GlcNAcylation. J Biol Chem. 2014 Oct 17;289(42):28816-26.
99: Karamanos NK, Passi A. Novel insights into matrix pathobiology regulatory mechanisms in health and disease. FEBS J. 2014 Nov;281(22):4978-9.
100: Karousou E, Asimakopoulou AP, Zafeiropoulou V, Viola M, Monti L, Rossi A, Passi A, Karamanos N. Fast screening of glycosaminoglycan disaccharides by fluorophore-assisted carbohydrate electrophoresis (FACE): applications to
biologic samples and pharmaceutical formulations. Methods Mol Biol. 2015;1229:143-59.
101: Vigetti D, Karousou E, Viola M, Passi A. Analysis of hyaluronan synthase activity. Methods Mol Biol. 2015;1229:201-8.
102: Moretto P, Karousou E, Viola M, Caon I, D'Angelo ML, De Luca G, Passi A, Vigetti D. Regulation of hyaluronan synthesis in vascular diseases and diabetes. J Diabetes Res. 2015;2015:167283.
103: Marcozzi C, Moriondo A, Solari E, Reguzzoni M, Severgnini P, Protasoni M, Passi A, Pelosi P, Negrini D. Regional lung tissue changes with mechanical ventilation and fluid load. Exp Lung Res. 2015 May;41(4):228-40. d
104: Viola M, Vigetti D, Karousou E, D'Angelo ML, Caon I, Moretto P, De Luca G, Passi A. Biology and biotechnology of hyaluronan. Glycoconj J. 2015 May;32(3-4):93-103.
105: Viola M, Karousou E, D'Angelo ML, Caon I, De Luca G, Passi A, Vigetti D. Regulated Hyaluronan Synthesis by Vascular Cells. Int J Cell Biol. 2015;2015:208303.
106: Uccella S, Cromi A, Vigetti D, Cimetti L, Deleonibus S, Casarin J, Passi A, Riva C, Ghezzi F. Endometrial cancer cells can express fibrinogen: Immunohistochemistry and RT-PCR analysis. J Obstet Gynaecol. 2016;36(3):353-8.
107: Kouvidi K, Berdiaki A, Tzardi M, Karousou E, Passi A, Nikitovic D, Tzanakakis GN. Receptor for hyaluronic acid- mediated motility (RHAMM) regulates HT1080 fibrosarcoma cell proliferation via a β-catenin/c-myc signaling axis. Biochim
Biophys Acta. 2016 Apr;1860(4):814-24.
108: Viola M, Karousou E, D'Angelo ML, Moretto P, Caon I, De Luca G, Passi A, Vigetti D. Extracellular Matrix in Atherosclerosis: Hyaluronan and Proteoglycans Insights. Curr Med Chem. 2016 Jun 6. [Epub ahead of print] PubMed PMID: 27281127.
109: Motolese A, Chiaravalloti V, Cerati M, Oldrini R, Mola F, Passi A. Interleukine levels and macrophagic density in periumbilical fat tissue in patients affected by moderate to severe psoriasis with metabolic syndrome, before
and after Etanercept treatment. G Ital Dermatol Venereol. 2016 Jun 10. [Epub ahead of print] PubMed PMID: 27284777.6)